draq5 (Miltenyi Biotec)
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draq5
Draq5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5/DRAQ5+Staining+Solution/pm41745542-139-33-34
Average 97 stars, based on 9 article reviews
Draq5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5/DRAQ5+Staining+Solution/pm41745542-139-33-34
Average 97 stars, based on 9 article reviews
draq5 - by Bioz Stars,
2026-10
97/100 stars
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Staining:Article Title: High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma. Article Snippet: Cells were detached with Trypsin-EDTA (Merck, Darmstadt, Germany) washed in PBS and fixed in 70% (vol/ vol) ethanol overnight at -20 °C, washed twice with PBS and incubated for 30 min with 50 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA). .. DNA was stained with 10 μM Article Title: Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior Article Snippet: After 48 h of incubation on test surfaces, M0-, M1-, and M2-MDMs were fixed with 3% paraformaldehyde (Sigma Aldrich Co., Steinheim, Germany; Cat. No. P6148) for 10 min at room temperature (RT), permeabilized with 0.2% Triton X-100 (Merck, Darmstadt, Germany; Cat. No. X100) in PBS for 10 min, and washed with PBS. .. Samples were blocked in 1% bovine serum albumin (BSA; VWR International, Leuven, Belgium; Cat No. 422361V) and 0.05% Tween-20 (Roth, Karlsruhe, Germany; Cat. No. 9127.1) in PBS for 30 min. Cytoskeletal staining was performed using 5 μg/mL phalloidin–FITC (Sigma Aldrich, Saint Louis, MI, USA; Cat. No. P5282) in blocking buffer for 1 h, followed by nuclear staining with 10 μM Article Title: Superhydrophilic Hierarchical Anatase Coating on Sandblasted, Acid-Etched Titanium: In Vitro Apatite Formation and Osteoblast Responses and the Role of Polar Surface Free Energy Article Snippet: Non-specific binding sites were then blocked with PBS containing 1% bovine serum albumin (VWR International, Leuven, Belgium) and 0.05% Tween-20 (Roth, Karlsruhe, Germany). .. The cells were subsequently stained with a 5 μg/mL FITC-Phalloidin conjugate solution (Sigma Aldrich, Saint Louis, MO, USA) for 1 h. Following three washes with PBS, the nuclei were counterstained with 10 μM Article Title: High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma Article Snippet: Cells were detached with Trypsin-EDTA (Merck, Darmstadt, Germany) washed in PBS and fixed in 70% (vol/vol) ethanol overnight at -20 °C, washed twice with PBS and incubated for 30 min with 50 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA). .. DNA was stained with 10 μM Article Title: Superhydrophilic Hierarchical Anatase Coating on Sandblasted, Acid-Etched Titanium: In Vitro Apatite Formation and Osteoblast Responses and the Role of Polar Surface Free Energy. Article Snippet: Non-specific binding sites were then blocked with PBS containing 1% bovine serum albumin (VWR International, Leuven, Belgium) and 0.05% Tween-20 (Roth, Karlsruhe, Germany). .. The cells were subsequently stained with a 5 μg/mL FITC-Phalloidin conjugate solution (Sigma Aldrich, Saint Louis, MO, USA) for 1 h. Following three washes with PBS, the nuclei were counterstained with 10 μM Article Title: Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior Article Snippet: After 48 h of incubation on test surfaces, M0-, M1-, and M2-MDMs were fixed with 3% paraformaldehyde (Sigma Aldrich Co., Steinheim, Germany; Cat. No. P6148) for 10 min at room temperature (RT), permeabilized with 0.2% Triton X-100 (Merck, Darmstadt, Germany; Cat. No. X100) in PBS for 10 min, and washed with PBS. .. Samples were blocked in 1% bovine serum albumin (BSA; VWR International, Leuven, Belgium; Cat No. 422361V) and 0.05% Tween-20 (Roth, Karlsruhe, Germany; Cat. No. 9127.1) in PBS for 30 min. Cytoskeletal staining was performed using 5 μg/mL phalloidin–FITC (Sigma Aldrich, Saint Louis, MI, USA; Cat. No. P5282) in blocking buffer for 1 h, followed by nuclear staining with 10 μM Article Title: High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma Article Snippet: Cell cycle analysis Cells were detached with Trypsin-EDTA (Merck, Darmstadt, Germany) washed in PBS and xed in 70% (vol/vol) ethanol overnight at -20°C, washed twice with PBS and incubated for 30 min with 50 μg/ml RNAse (Thermo Fisher Scienti c, Waltham, MA, USA). .. DNA was stained with 10 μM Flow Cytometry:Article Title: High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma. Article Snippet: Cells were detached with Trypsin-EDTA (Merck, Darmstadt, Germany) washed in PBS and fixed in 70% (vol/ vol) ethanol overnight at -20 °C, washed twice with PBS and incubated for 30 min with 50 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA). .. DNA was stained with 10 μM Article Title: High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma Article Snippet: Cells were detached with Trypsin-EDTA (Merck, Darmstadt, Germany) washed in PBS and fixed in 70% (vol/vol) ethanol overnight at -20 °C, washed twice with PBS and incubated for 30 min with 50 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA). .. DNA was stained with 10 μM Blocking Assay:Article Title: Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior Article Snippet: After 48 h of incubation on test surfaces, M0-, M1-, and M2-MDMs were fixed with 3% paraformaldehyde (Sigma Aldrich Co., Steinheim, Germany; Cat. No. P6148) for 10 min at room temperature (RT), permeabilized with 0.2% Triton X-100 (Merck, Darmstadt, Germany; Cat. No. X100) in PBS for 10 min, and washed with PBS. .. Samples were blocked in 1% bovine serum albumin (BSA; VWR International, Leuven, Belgium; Cat No. 422361V) and 0.05% Tween-20 (Roth, Karlsruhe, Germany; Cat. No. 9127.1) in PBS for 30 min. Cytoskeletal staining was performed using 5 μg/mL phalloidin–FITC (Sigma Aldrich, Saint Louis, MI, USA; Cat. No. P5282) in blocking buffer for 1 h, followed by nuclear staining with 10 μM Article Title: Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior Article Snippet: After 48 h of incubation on test surfaces, M0-, M1-, and M2-MDMs were fixed with 3% paraformaldehyde (Sigma Aldrich Co., Steinheim, Germany; Cat. No. P6148) for 10 min at room temperature (RT), permeabilized with 0.2% Triton X-100 (Merck, Darmstadt, Germany; Cat. No. X100) in PBS for 10 min, and washed with PBS. .. Samples were blocked in 1% bovine serum albumin (BSA; VWR International, Leuven, Belgium; Cat No. 422361V) and 0.05% Tween-20 (Roth, Karlsruhe, Germany; Cat. No. 9127.1) in PBS for 30 min. Cytoskeletal staining was performed using 5 μg/mL phalloidin–FITC (Sigma Aldrich, Saint Louis, MI, USA; Cat. No. P5282) in blocking buffer for 1 h, followed by nuclear staining with 10 μM other:Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation Article Snippet: Ovaries from ~1-week-old adult female flies ( Drosophila melanogaster ) were dissected in Grace’s insect cell culture medium (Gibco, Gaithersburg, MD, USA), fixed in 4% paraformaldehyde for 15 min and then washed three times in 1X PBST for 5 min. Cytometry:Article Title: High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma Article Snippet: Cell cycle analysis Cells were detached with Trypsin-EDTA (Merck, Darmstadt, Germany) washed in PBS and xed in 70% (vol/vol) ethanol overnight at -20°C, washed twice with PBS and incubated for 30 min with 50 μg/ml RNAse (Thermo Fisher Scienti c, Waltham, MA, USA). .. DNA was stained with 10 μM |
![(A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with <t>Draq5</t> (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_37/10__64898_slash_2026__02__16__706237/10__64898_slash_2026__02__16__706237___F1.large.jpg)